Lung Epithelial Cell Line A549

A549 cell line origin.

Lung epithelial cell line a549.

Human caucasian lung carcinoma cell line description derived from a 58 year old caucasian male. In a study to. Ccl 185 a549 lung carcinoma homo sapiens 58 years caucasian the cells are positive for keratin by. A549 cells were cultured in kaighn s modification of ham s f 12 f 12k medium atcc nci h23 nci h1299 and hcc827 cells were cultured in rpmi 1640.

20 mm and 10 fetal calf serum at 37 c. Through the removal and culturing of cancerous lung tissue in the explanted tumor of a 58 year old caucasian male. Cell culture and seeding of a549. This adenocarcinomic cell line is categorized as a non small cell lung carcinoma nsclc which tends to.

Human lung epithelial cells a549 which were bought from shanghai institutes for biological sciences. The cells can synthesise lecithin utilising the cytidine diphosphocholine pathway. Giard et al through an explant culture of adenocarcinomic lung tissue of a 58 year old caucasian male. The cell line of a549 was cultured in atcc formulated modified f 12 k medium containing 10 fetal bovine serum 100 u ml penicillin and 100 u ml streptomycin sulfate.

A549 cells are hypotriploid alveolar basal epithelial cells. A continuous tumor cell line from a human lung carcinoma with properties of type ii alveolar epithelial cells. Cell culture nsclc cells a549 nci h23 nci h1299 and hcc827 and human normal lung epithelial cells beas 2b were bought from american type culture collection atcc manassas va usa. The a549 cell line was established in 1972 by d j.

X54079 human mrna for heat shock protein hsp27. Cross references nucleotide genbank. The a549 cell line is a suitable host for many human. A549 cells are adenocarcinomic human alveolar basal epithelial cells and constitute a cell line that was first developed in 1972 by d.

Application enzymatic studies human a549 cell line has been. The a549 cell line was isolated in 1973 from a pulmonary adenocarcinoma. Lung cancer and normal cell lines. The cells are used as models for the study of lung cancer and the development of drug therapies against it.

Occasional cells may also contain inclusion bodies although they are not known to carry any human pathogen. Cancer research the generation of epithelial models of thedistal lung for studies into airway function and disease and virology. A549 a cell line model of nsclc used to evaluate the role of ccl22 and il 37 in epithelial mesenchymal transition 3 toxicology studies cellular toxicity of carbon based nanomaterials was tested in calu 1 and other lung cancer cell lines 4.